饲粮蛋氨酸对鹅再生羽绒品质、血清指标及基因mRNA表达量的影响

doi:10.11843/j.issn.0366-6964.2019.02.010

Abstract

Abstract:

The aim of this study was to investigate the effects of dietary methionine on the regeneration performance of goose down, serum indexes and the expression of genes related to cysteine and methionine metabolic pathways in Hortobagy goose (Goosebag goose). One hundred and twenty eight 150-day-old healthy Hortobagy geese were selected and randomly divided into 4 groups after epilation. Four replicates for each group, and 8 geese for each replicate. Geese in the 4 groups were fed diets with additional methionine levels of 0, 0.28%, 0.56% and 0.84%, respectively. The formal experimental period was 42 days. The data of goose down such as the thousand plush weight,velvet length, serum biochemical indicators, growth factors and antioxidant factors levels were measured, and the expression levels of genes related to cysteine and methionine metabolism pathways were determined by RT-qPCR. The results showed as the following:1) There was no significant difference in the TP,ALB,BUN,TG,HDL and LDL levels in serum among different groups (P>0.05). At the same time, with the increase of dietary methionine concentration, the growth factor (IGF-1), prolactin(PRL) and the glutathione (GSH-Px) levels in serum were also significantly increased(P<0.05), but when the methionine level added to 0.84%, the concentration of the above factors reduced. 2) Supplementation of methionine in diet could significantly increase the thousand down weight and velvet length of back(P<0.05), and promote the regeneration of down, but there was no significant difference in the yield of down among the different methionine supplementation groups(P>0.05). 3) The results of RT-qPCR showed that, with the methionine addition, the mRNA expression of MAT1A gene in cysteine and methionine metabolism pathway decreased significantly (P<0.05), and the expression of ACHY mRNA extremely significantly decreased in the 0.28% methionine supplementation group(P<0.01). The results indicate that suitable concentration of methionine in diet can promote the secretion of IGF-1 and PRL growth factors and antioxidant factors in goose. It can also significantly increase the thousand plush weight and velvet length of back, reduce the mRNA expression of the MAT1A and ACHY genes in cysteine and methionine metabolism pathways and promote the regeneration of the down feather, and the suitable total addition level is 0.55%-0.83%.

CLC Number:

S835.5

WANG Huiying, GUO Baodi, HE Daqian, GONG Shaoming, LIU Yi, LI Guangquan, XU Qi, CHEN Guohong. Effect of Dietary Methionine on Down Quality of Regeneration, Serum Indexes and Gene Expressions in Goose[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 323-331.

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Effect of Dietary Methionine on Down Quality of Regeneration, Serum Indexes and Gene Expressions in Goose

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